Random Mitotic Activities Across Human Embryonic Stem Cell Colonies
نویسندگان
چکیده
منابع مشابه
Clonal neural stem cells from human embryonic stem cell colonies.
Clonal cell culture is crucial for experimental protocols that require growth or selection of pure populations of cells. High-density derivation of neural progenitors from human embryonic stem cells (hESCs) can lead to incomplete differentiation, and transplantation of resulting heterogeneous cell mixtures can cause proliferation of tumorigenic clusters in vivo. We have identified the neural pr...
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We perform a detailed analysis of the migratory motion of human embryonic stem cells in two-dimensions, both when isolated and in close proximity to another cell, recorded with time-lapse microscopic imaging. We show that isolated cells tend to perform an unusual locally anisotropic walk, moving backwards and forwards along a preferred local direction correlated over a timescale of around 50 mi...
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MATERIALS Reagents AmpliTaq polymerase (Perkin Elmer) Bovine serum albumin (BSA) (nuclease-free; Boehringer Mannheim; stock 1.6 mg/ml) Dimethyl sulfoxide (DMSO) (Aldrich) DNA in ES PCR Lysis buffer (include positive and negative controls) dNTPs (Pharmacia; stock of the mix 10 mM) ES colonies in G418 selection (see Electroporating DNA into Embryonic Stem (ES) Cells and Selection) Light mineral o...
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Identification and quantification of the characteristics of stem cell preparations is critical for understanding stem cell biology and for the development and manufacturing of stem cell based therapies. We have developed image analysis and visualization software that allows effective use of time-lapse microscopy to provide spatial and dynamic information from large numbers of human embryonic st...
متن کاملHuman embryonic stem cell therapy.
The author presents an overview (completed on September 15, 2001) of three issues involved in the ethics of human embryonic stem cell therapy: the ethical implications of some of the scientific issues involved, the specific ethical issues of the moral standing of the early human embryo and the problem of cooperation, and a consideration of two public policy issues: should the research go forwar...
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ژورنال
عنوان ژورنال: Stem Cells and Development
سال: 2010
ISSN: 1547-3287,1557-8534
DOI: 10.1089/scd.2009.0324